Amir Mohammed Alsharabasy

12 Questions 53 Answers 0 Followers

Questions related from Amir Mohammed Alsharabasy

A mixture of DMSO, WATER and ETHER. What are the effects of miscibility on material purification?

I am trying to extract my products from DMSO without using the evaporation method. I tried mixing with water, then ether, and succeeded in the purification by filtration; this method has been...

01 January 2019 3,991 8 View

Can the stability test in Gaussian-DFT give indication to the global minimum point?

I am performing the frequency calculation for some of my molecules, and gradually optimized the structures till the final optimization using the basis set 6-311+G(d,p) and [opt=tight], then...

01 January 2019 5,622 4 View

A problem with seeding fibroblasts in collagen hydrogel?

Previously, I used to seed primary dermal fibroblasts (which initially had slow growth rate) in collagen hydrogel and the cells used to show complete attachment and the normal shape of fibroblasts...

10 October 2018 9,308 5 View

Why did the protein concentration of a hemolysate increase?

I am trying to purify a stroma-free lysed RBCs for hemoglobin isolation using 14 KDa dialysis bags. Using BCA assay for total protein quantification before the SDS-PAGE, I got the following...

03 March 2018 1,614 4 View

What may be the problem with these chromatograms?

I am analysing isolated heme from blood using HPLC (Mobile phase: Methanol:H2O: Acetic acid) with identification at two different wavelengths. The chromatograms at 405 and 500 nm are shown. I...

03 March 2018 7,423 16 View

Bradford assay or BCA assay for protein quantification?

Following the digestion of decellularized tissue using pepsin, which is a better method for the quantifying the released proteins to estimate the rate of reaction; Bradford assay or the BCA assay?

11 November 2017 3,319 3 View

Can the extracellular matrix from bovine urinary bladder be used for hydrogel synthesis?

I am trying to make a hydrogel from the bovine Urinary Bladder Matrix (UBM), but can't get any hydrogel from the digested matrix using pepsin. Despite the complete digestion of both 10 and 20 %...

10 October 2017 3,397 2 View

How can we concentrate a partitioned protein solution?

Using PEG/salt aqueous two phase extraction system, after the partitioning of protein to either the polymer or salt phase, which are the suitable methods for concentrating of these proteins? Any...

10 October 2017 925 15 View

What is the problem with this agarose gel electrophoresis image?

I am studying the release of DNA from nanoparticles in acidic medium at different times (lanes 4,5,6,7,8 from the left), but no bands appear in the gel in contrary to the main DNA appeared in...

05 May 2017 2,410 34 View

What is the Hydrated Density?

I measured the true density of porous bone samples by a helium pycnometer, and calculated the apparent density, but in one of the papers, the measured bone density is called hydrated density which...

02 February 2017 245 0 View

Should I ask for reproduction permission if an article is distributed under the Creative Commons Attribution License?

I am preparing a report and want to use some figures from some papers, and wish to know if I have to take the permission of the author, the journal or the publisher before using it as it is. Some...

12 December 2016 9,313 2 View

Can the polymer have kosmotropic properties?

i am working on Polyethylene glycol/ sulfate aqueous two phase partitioning, but with increasing the concentration of the salt (below its critical concentration), crystallization happens, in...

01 January 1970 5,004 2 View