Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View