What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

02 January 2021 3,352 6 View

How can we know about the final concentration of each component to be used in the master mix for the PCR ?

Final concentration for each component in master mix depends on what or how it will be known that in this master mix the concentration required is __ microliters ?

02 January 2021 5,764 2 View

How can I separate features and the background to measure pixel intensity?

For image processing, I am using ImageJ. I need to separate the features from the background and calculate pixel intensity of features and background. Can anybody help me in this? Thanks

09 December 2020 7,472 9 View

Which are the appropriate metrics can be used to calculate overall Precision, Recall and F-measure in different data samples? and when can use F1?

If we have three different domain of data (e.g. security, AI and sport) and we did 3 different case study or experiments (1 for each domain) and we estimate the Precision, Recall and F-measure for...

07 December 2020 9,833 3 View

How can stem cells repair damaged cells?

How can stem cells repair damaged cells? Could anyone provide papers show the therapeutic potential of stem cells in vitro (on the disease model)?

29 November 2020 9,534 6 View

How can to calculate the Computational complexity/cost (espatially, number of operations and space/memory cost) for an algorithm?

As we know, computational complexity of an algorithm is the amount of resources (time and memory) required to run it. If I have algorithm that represents mathematical equations , how can estimate...

25 November 2020 6,950 7 View

Which is the best scale available to measure conflict management styles?

I have come across multiple instruments for conflict. Now I'm confused as to which one to use. Expert opinions are welcome.

22 November 2020 3,651 5 View

Chi square test for the over time tested variable ?

16 November 2020 8,291 4 View

Help me find the transfer function of the following non linear coupled ODE system ?

I need to find the transfer function for the system in MATLAB. I do know the ss2tf command but its not that simple. Please guide.

08 November 2020 4,414 1 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View