usually students ask about the ideal method for primary assement methodof anticancer ar antitumor activity of the natural agents
MTT assay or CCK8 is usually first used to detect whether a natural agent can inhibit the growth of some cancer cell line in vitro, which is the first step to determine the anticancer activity of some crude extract.
MTT assay is most widely used method. Once it is done, you should proceed to cancer cell lines.
You can choose various cancerous cell line for your MTT assay to exactly pin point the anti-tumor activity.
Hi Mona,
MTT assay in EMLA cells may be an useful method.
demso also give -ve result for the biological assay
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