How do you determine the upper detection limit for E. faecium?
If there are too many to count on the plate, can I suggest that there are >25,000 CFU/ml for example? - or would that be too much
There does not exist upper limit. You can perform dilution if necessary. So even if the count is very high, you can perform dilution as you need without upper limit.
Best regards
Vit
Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?
01 February 2021 5,965 1 View
I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?
04 October 2020 5,777 1 View
Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...
29 August 2020 1,384 4 View
Do you first calculate the average of all the CFU and then sub it into the equation ? OR Do you calculate each individual log CFU/ml and then average that?
19 March 2019 7,956 6 View
I have a treated and a control condition of agar plates. I counted the number of colonies and wanted to carry out a mann whitney test. Do I carry out the mann whitney test using the counted...
02 March 2019 4,830 1 View
02 March 2019 1,412 7 View
For example, do isopropanol/1-propanol/ethanol alcohol based hand rubs all use the same mechanisms to kill bacteria? Do they all just disorganize lipid membranes, denature proteins, and...
02 March 2019 8,814 3 View
I'm trying to use homology modelling to find the structure of the N-terminus of a protein which is thought to contribute to its hetero-oligomeric structure. However, I can't find an x-ray...
24 December 2018 6,810 3 View
Zone of inhibitions sometimes appear fuzzy/sharp with different antibiotic discs on the same agar plate, what are the explanations for this? Also, how important are these when determining the...
10 November 2017 8,676 0 View
Why is that a higher affinity leads to increased effectiveness of a drug inhibitor? Also, most importantly why do enzymes bind more tightly to transitions state analog inhibitors? what is...
11 December 2016 2,057 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View