How to determine if mean evolutionary rate (MER)computed by MEGA is statistically significant?

I used MEGA5.0 for computing MER for my dataset. the rates were computed based on JTT with an account of invariant sites model using 2 gamma categories for all positions in the alignment. these...

07 August 2018 5,351 1 View

Is it possible to remove just one node from a phylogenetic tree without actually making the tree again?

31 December 2016 3,840 7 View

Is it possible to express and purify proteins in large quantities from mammalian cell lines ?

generally bacteria is used for protein expression in purification whenever protein is required in large quantities for structural studies. Is it possible to get such large amounts of proteins from...

07 August 2016 6,503 7 View

Can one replace one cloned shRNA with the other from pSUPER retro puro vector by merely removing it by using modified restriction cloning sites?

I have a pSUPER retor puro vector that already has a cloned shRNA. I wanted to remove the already cloned shRNA and replace it with the other shRNA. the user manual from oligoengine sys that upon...

06 July 2016 4,061 4 View

How long does one need to incubate samples for digestion with dpn1?

10 November 2015 6,378 4 View

How can I optimize antibiotic concentrations for generating a gene of interest expressing stable cell line?

I have my gene of interest cloned in mammalian expression system. there are two gene that i am looking for. i have cloned each of them in two different plasmids both having different promotors of...

05 June 2015 1,071 3 View

How does one select the best fit model for constructing phylogenetic trees?

I am working on mitofusins which are of two types in humans (not isofoms, but two different genes encoding them). i want to know at what time in evolution did the duplication happen. also which...

05 June 2015 4,956 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View