How long does one need to incubate samples for digestion with dpn1?

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Elbadri E. Osman

For most incubation temperatures, I think 20 minutes are good

Dragana Matekalo

From which supplier is the enzyme?

Michael J. Benedik

There is no simple answer because the amount of time depends upon the amount of enzyme and the amount of target DNA. So the reaction could be completed in seconds or you might need to let it go for some hours, depending upon those factors.

Romain Dumoulin

It depends. But for example, to digest 10ng of parental DNA, Agilent (in their QuickChange kit) says 10U of DpnI @ 37°C during 1 hour.