How to determine if mean evolutionary rate (MER)computed by MEGA is statistically significant?

I used MEGA5.0 for computing MER for my dataset. the rates were computed based on JTT with an account of invariant sites model using 2 gamma categories for all positions in the alignment. these...

07 August 2018 5,351 1 View

Is it possible to remove just one node from a phylogenetic tree without actually making the tree again?

31 December 2016 3,840 7 View

Is it possible to express and purify proteins in large quantities from mammalian cell lines ?

generally bacteria is used for protein expression in purification whenever protein is required in large quantities for structural studies. Is it possible to get such large amounts of proteins from...

07 August 2016 6,503 7 View

Can one replace one cloned shRNA with the other from pSUPER retro puro vector by merely removing it by using modified restriction cloning sites?

I have a pSUPER retor puro vector that already has a cloned shRNA. I wanted to remove the already cloned shRNA and replace it with the other shRNA. the user manual from oligoengine sys that upon...

06 July 2016 4,061 4 View

What is the general cut off used in CD-HIT when using a data set of closely related sequences for phyognetic analyses?

my dataset consists of highly similar sequences of proteins. i only wanted to remove duplicate sequeces if any in the data set. what threshold shall I set in CD HIT?

03 April 2016 5,448 1 View

How can I optimize antibiotic concentrations for generating a gene of interest expressing stable cell line?

I have my gene of interest cloned in mammalian expression system. there are two gene that i am looking for. i have cloned each of them in two different plasmids both having different promotors of...

05 June 2015 1,071 3 View

How does one select the best fit model for constructing phylogenetic trees?

I am working on mitofusins which are of two types in humans (not isofoms, but two different genes encoding them). i want to know at what time in evolution did the duplication happen. also which...

05 June 2015 4,956 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View