I'm running the influenza HI assay on influenza virus a/california/07/09pdm using typo-0 human rbcs. I have 2300 sera to get through. All have been RDE treated to rid of inhibitors but I want to avoid if possible treating all with RBC adsorption to remove any potential NSAs.
Does anybody know roughly the percentage of samples that might be expected to contain NSAs, are they common
Also if I do test for them, is it necessary to look for agglutination in all the 2-fold dilutions or can I just look for agglutination in the 1:40 dilution of rde treated sera.
Cheers
Mark
we always run a PBS control as one of the antigens using the sera. That way you can see if there are any non-specific agglutinins left in your sera following treatment.
Thanks Thomas
Can i just perform the PBS control on a 1:40 dilution of the serum rather that testing the whole 2-fold dilution series for every sera?
Should work. We normally just use PBS as an additional antigen so that we can compare directly when we perform a test. We usually run about 100+ viruses for each HI test with 8-16 sera.
A PBS control can be run as antigen by using the sera and can be looked for the presence of non-specific agglutinins left in the sera (followed by treatment).
Mark - I am sorry, but HI test is so "must see plate" - Tom Rowe is expert
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs