Mark Quinlivan

11 Questions 37 Answers 0 Followers

Questions related from Mark Quinlivan

Clone mRNA as cDNA clone or whole gene?

Hi Guys & Girls This is a bit of an amateurish question but if I want to expresss mouse zinc finger protein335 using plasmid pcDNA3.1, should I clone the cDNA of the mRNA (~4587bp) or the...

24 January 2017 8,328 1 View

What is the frequency of non-specific agglutinins in human sera?

I'm running the influenza HI assay on influenza virus a/california/07/09pdm using typo-0 human rbcs. I have 2300 sera to get through. All have been RDE treated to rid of inhibitors but I want to...

25 June 2015 4,257 6 View

Can someone help with the interpretation of Influenza haemagglutination inhibition assay results?

Hi Guys I`m in need of your opinions on interpretation of HI resuts I`ve attached a couple of annotated plates where I seem to have four different outcomes. Could you read them and say if you...

20 June 2015 3,819 8 View

Any advice on the choice of v-bottom vs U-bottom 96 well plate for influenza HI assay?

I`ve read some studies using V-bottom plates for avian rbcs and U-bottom for human. I understand human sink slower than avian. But I see the same results using human rbcs in both well types....

20 June 2015 7,587 7 View

Can anyone help with removal of non-specific agglutinate for influenza haemagglutinaion inhibition assay?

Hi guys I'm running the HI assay on 2300 sera using A/California/07/09pdm as the antigen and type0 human rbcs. All sera have been rde treatedbut is itnecessary torrent all by adsorptionwith rbcs...

16 June 2015 6,198 2 View

Any suggestions on lysis versus genuine pellets in the Influenza HI test?

I finding convincing looking pellets/buttoning of RBCs in some wells but they dont stream upon tilting compared with others that clearly do stream. They form at the same time as the pellets that...

04 June 2015 6,656 1 View

Why does my dmem turn purple?

I'm having a similar problem. I buy in dmem, the cells look good but the media changed colour to pinkish purple within hours. It looked a bit cloudy but I couldnt see anything unusual under the...

15 May 2015 7,993 9 View

Can someone help regarding RBC pelletet in the influenza haemagglutination inhibition assay?

Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the...

13 May 2015 6,008 3 View

What I should use to dissolve TPCK powder to be used in DMEM to culture Influenza virus?

Can anyone tell me what I should use to dissolve TPCK powder to be used in DMEM to culture Influenza? I`ve been using 100nM tris-HCL and just wanted to che ck this is correct Cheers

13 April 2015 1,536 7 View

Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm?

Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm. I will pay any costs. I do have some but I`m getting poor virus titres and have...

05 April 2015 7,160 3 View

Any suggestions on the inactivation and purification of Influenza for Haemagglutination inhibition assay?

Guys please help me out. I was given the protocol below for inactivation of FLU A Pandemic 2009 H1N1 for the HA assay but I get no agglutination on the HA assay. For step 1. I take 4ml of...

04 March 2015 1,353 18 View