What is the Fold changes ?

Hassan Nima @Hassan_Nima2

01 January 2018 0 4K Report

gene expression

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How to convert a Text File to a Fasta Format File?

10 November 2018 3,936 0 View

Which came first, the German Peasants War or the English Peasants Revolt?

German Peasants War, English Peasants Revolt

05 June 2018 7,031 1 View

Why the DNA considered a genetic material ?

DNA , genetic material

04 May 2018 9,780 0 View

Which is the most credible free online IQ test?

credible free online IQ test

04 May 2018 3,605 1 View

Are there any animals that actually smile when they're happy ?

animals that actually smile when they're happy

04 May 2018 9,762 0 View

Is an offline IQ test more reliable than a online IQ test?

offline IQ , online IQ test

04 May 2018 2,624 1 View

At the age of 28, is it too late to start a PhD in the biotechnology field or other ?

the age of 28, start a PhD

03 April 2018 3,887 98 View

What is the best way to extract DNA from fungi ?

extract DNA, fungi

02 March 2018 4,916 3 View

If the bacteria multiply all their genes within 36,000 minutes, how many genes have multiply within one hour ?

gene multiply

02 March 2018 4,777 0 View

Staying in space for a long time affects DNA or gene expression ?

space , DNA, gene expression

02 March 2018 6,631 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View