Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Summer Studentship opportunities in Analytical Chemistry LC-MS HPLC Chromatography?

The ChromSoc/BMSS 2021 Summer Studentship Applications are now OPEN (DEADLINE 15th JAN). ChromSoc & BMSS , annually funds research projects to take place over the summer . The scheme provides...

17 December 2020 5,535 3 View

How do make a horizontal Boxplot incl. Outliers? (Excel, SPSS, others)?

I have recorded a time series (in minutes + seconds) and would like to make a horizontal boxplot. I have vertical boxplots in SPSS and Excel. However, Excel is not able to make horizontal boxplots...

30 November 2020 9,005 6 View

Has anyone generated Flp-In host cell lines from HeLa TRex cells? If so, anyone willing to share a detailed protocol ?

I am trying since 2 months to generate HeLa Flp-In TRex cell lines, but failed. If someone have a detailed protocol, including cell number to seed, transfection method used, antibiotic...

22 November 2020 5,723 3 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Statistics on prevalence of methodological approaches in psychological research?

I am currently looking for data on psychological research. I am interested in -current and previous- prevalence of methodological approaches in psychological research (e. g. the proportion of...

15 November 2020 5,711 3 View

Does Formic Acid linger in the MS?

Does anyone know if formic acid has a tendency to linger in the MS after organic solvent cleaning? If it does, are there any special processes that can be put in place to remove it? Thanks!

11 October 2020 3,828 2 View

SPSS frequencies bar chart?

Hello, is it possible to get a bar chart in SPSS where I have the frequencies on the y-axis and two variables on the x-axis? How do I get the two variables on the x-xis? thank you

01 October 2020 6,876 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View