What is the fate of a vector backbone once the gene of interest gets integrated in a plant genome?

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Which is best kit to study the interaction of protein and RNA from tissue sample?

Hello, I am interested in studying the interaction between RNA and proteins, specifically focusing on a particular protein and its corresponding regulatory gene, in mice aortic tissue. Could you...

28 November 2023 4,055 0 View

How to correct error in MO analysis of a dimer in Gaussian ?

In Gaussian, I am trying to do the MO analysis of a compound which is two metal centered having same organic moieties on both the metal. But, I am getting the LUMO which is appearing on only one...

24 January 2023 2,930 2 View

Hello Researchers, is there any alternative for MinPET software for processing of EPMA data?

MinPET software works only below win7 32 bit operating system. So we are unable to use it in higher operating system.

18 October 2022 394 0 View

Flea Beetles control in Cauliflower?

Dear connection, I need your help reagarding 100% control of Flea Beetles in Cauliflower crops. Thanks and regards Dhananjay Yadav

24 January 2022 9,539 3 View

How do we identify a mineral using SEM-EDX data i.e elements in wt%. Is there any atlas, EDX- library or guide for mineral identification?

Data is provided in terms of wt% and Atomic wt% of elements. So how do we identify the mineral?

08 September 2021 3,608 1 View

Can somebody suggest any software related to Laser parameteric analysis?

I would like to understand the different parameters of laser (pulsed laser) and their effect. In my case laser specification is a Q-switched Nd: YAG laser (200 mJ; 30 Hz; 6-9 ns). I am using this...

12 August 2021 8,550 3 View

Does anyone have state estimation MATLAB code for 3-phase unbalanced distribution network?

Does anyone has state estimation code using WLS for a 3-phase unbalanced IEEE 13 bus system. Is there any relation with r/x ratio to its convergence. Please help me. Thank you in Advance.

25 June 2021 8,838 1 View

How do you go about isolating the source of electrical noise on a ephys rig?

I am very new to electrophysiology. I have tried to the model cell and the noise does not go away. I have foiled all the wires that come out of the rig. I have tried unplugging and replugging the...

09 June 2021 5,162 1 View

In distribution system what are the methods which can be applied for finding Fault Location?

Using SCADA system what data parameters and best method (formula) may be utilized for fault location.

30 May 2021 7,415 9 View

How to modify the Y/Z bus matrix for 3 phase lines between two bus and how to do load flow analysis in distribution system?

How the distribution system power flow analysis is done for each phase of the line including various components of distribution system? Thank you in advance for your help.

31 August 2020 2,447 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View