Happy New Year.... Please can anyone help me out to find "How can I extract Amino acids from chickpea leaf sample for estimation by UPLC?" I want to quantify branched chain amino acids leucine, isoleucine and valine.
How the slurry is preparing for cathodes in batteries?
13 June 2024 4,314 3 View
Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.
09 June 2024 1,805 4 View
How can analyses a split plot design by SPSS?
02 June 2024 5,946 2 View
What do you mean by meadow plant?
01 June 2024 7,248 2 View
In any professional education students' Competency is Evaluated and used as the measurement of Outcome of the teaching-learning process. Then how these two are differentiated in curriculum...
22 May 2024 8,488 25 View
I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...
19 May 2024 7,170 1 View
I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...
17 April 2024 8,025 6 View
i have performed simulation in two steps and I wasn't able to merge the energy files. Provide help
16 April 2024 8,524 2 View
Hello there! I am running transient CFD simulation for a vehicle ideal gas law and the energy equation and I seem to be getting a negative gauge static pressure in the whole domain, even though...
19 March 2024 629 2 View
Hi, I am working on subpixel level measurement of deformation using FPGA with the help of image processing technique. Can anybody suggest me the steps to be followed? As per citation pixel level...
14 March 2024 4,078 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View