RABD PCR

Hi 

Maybe contamination. Try to performance a new pcr with new products. What kind of tissue are you use?

Regards

Hi,

Its seem non specific amplification due to following reason:

(1) Nonspecific amplification due to too much template

(2) Many time I get same result when I amplify insert from plasmid having the insert due to low annealing temp.

(3) Use gradient PCR from annealing temp Tm-5 to Tm+5. many time using annealing temp more then Tm will surprisingly gives good specific amplification.

(4) Check primers for their binding specificity.

You can find a lot literature on PCR troubleshooting.

Hope this will help.

thnx

First my question what is the size of your product (how many base pairs).

Non specific amplification. Mention your PCR conditions and components. 

As mentioned above try gradient. (If you have patience read my PCR answers I answered many). 

Non specific and fragmented DNA. Clean up your DNA, increase annealing temp of primers and do titration of magnesium in your pcr mix

Non-specific amplification. I think if you increase magnesium concentration and annealing temperature, you can get rid of most of the background. Also, include positive and negative controls.

Need more information. What exactly are you trying to amplify and what running conditions you used? It can be a perfectly normal result....

I would say not only non specific amplification, but random amplification (which may be non enzymatic).

With my pleasure to you sir .

But I have not good information and poorly  about this advance technique ?

take my respects .

RABD PCR

I too fully agree with Rana......

Dear Dr. Hussein Oleiwi Muttaleb Al-Dahmoshi ,

PCR is a technique used in the lab to make millions of copies of a particular section of DNA. It was first developed in the 1980s. 

https://www.yourgenome.org/facts/what-is-pcr-polymerase-chain-reaction

Regards,

Shafagat

Non specific amplification. Try to increase anneling temperatures, change magnesium concentration. Try to use more purified DNA without contaminations. Perform in non contaminated area. Decrease number of cycles.

I think that the primer specificity is not that good. But it would be easy to analyze the actual cause if you provide the pcr condition. I think you can use a gradient pcr to standardize the anealing temperature. Also use the primer last tool of ncbi to check the secondary targets of these primers.

RABD pcr picture

Specificity of primer or use of impure gel during electrophoresis

I think rapid pcr

RABD PCR

may be RABD PCR or non specific primers