Pseudomonas aeruginosa Have low percent Of biofilm Formation Although It's Clinical Isolated From sputum And Pus .
What Is The Explaination Of This Low Percent ?
P. aeruginosa produces stable biofilms, which is one of the reasons why it's so hard to treat with antibiotics. You should specify your question. If you cannot produce biofilms with aeruginosa, you should change your conditions
I think this might attribute to nutritional or physical factors
The ability to form biofilm of Pseudomonas aeruginosa can be variable. It depends e.g. on the surface used (e.g. polistyrene plate), medium change and its composition (should be changed every 12 hours), and space (biofilm formation may be diminished by too high density of planctonic cells. Biofilm formation is strongly associated in pseudomonads with QS system. Maybe your isolate is some sort of mutant in terms of QS? Generally pseudomonads produce biofilms efficiently. Are you sure that your isolate is Pseudomonas aeruginosa? For example Burkholderia cepacia is very similar to P. aeruginosa, although both can produce biofilms.
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