Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

01 February 2020 9,732 3 View

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01 February 2017 1,213 5 View

What statistical technique I use and how I use it to analyze a comparison between a native strain and control strain for the solubilization ofcopper?

evaluated the bioleaching of a native strain compared with a control strain, have variables (cell concentration,% solubilization of copper, time) and the taking of the data is that starting from a...

03 April 2016 8,586 1 View

Is it effective to determine iron II with potassium dichromate in the presence Iron III?

10 November 2014 5,146 13 View

When using heap leach or stirred tank bioreactors, does it depend on the mineral to use?

There are many processes / patents using bioleaching of sulphide ores or oxidized copper / gold, using both batteries as bioreactors, but this will depend on that? The percentage of precious metal...

05 June 2014 9,514 4 View

Can I concentrate microbial biomass without centrifugation or using membrane filters?

In the lab I work at we do not have many tools for microbiology.

03 April 2014 7,591 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View