Luis A. Salcedo Mejía

7 Questions 13 Answers 0 Followers

Questions related from Luis A. Salcedo Mejía

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

02 February 2020 9,826 3 View

The incubation with isopropanol to -20°C overnight increase the ARN recuperation?

Hi all, I did read few papers that write protocols with overnight incubation with isopropanol for increase RNA recuperation, but the handbook (f.e. qiazol) does not mention it, only an incubation...

02 February 2017 1,279 5 View

What statistical technique I use and how I use it to analyze a comparison between a native strain and control strain for the solubilization ofcopper?

evaluated the bioleaching of a native strain compared with a control strain, have variables (cell concentration,% solubilization of copper, time) and the taking of the data is that starting from a...

04 April 2016 8,684 1 View

What is the exactly definition of metagenomics??

I work in Biomining and have a question.Performed from a liquid culture using molecular identification 16S rRNA is metagenomics? thank you very much!!

02 February 2015 7,469 1 View

Is it effective to determine iron II with potassium dichromate in the presence Iron III?

I realize experiments of bioleaching of polymetallic ores with bioleaching consortia, and want to determine iron (II). My protocol is: To 1mL sample (aprox pH 2- 5) add 1mL solution H3PO4:H2SO4...

11 November 2014 5,226 13 View

When using heap leach or stirred tank bioreactors, does it depend on the mineral to use?

There are many processes / patents using bioleaching of sulphide ores or oxidized copper / gold, using both batteries as bioreactors, but this will depend on that? The percentage of precious metal...

06 June 2014 9,573 4 View

Can I concentrate microbial biomass without centrifugation or using membrane filters?

In the lab I work at we do not have many tools for microbiology.

04 April 2014 7,680 1 View