Please suggest me the simplest way to understand the in-silico analysis of any gene?

I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?

22 February 2021 8,879 2 View

Salt aggregration during Gromacs simulation at oil-water interface?

Hi I am simulating a bilayer structure of decane oil-water with AOS surfactant presence at the interface. The middle water layer has a NaCl concentration of approximate 12.11 wt%. Force fields...

15 September 2020 5,248 4 View

Is there anyone who can help for English language editing of my manuscript?

Hello Sir/Ma'am, I am Dr Kamlesh Kumar Maurya from India, Sir/Ma'am one of my manuscript is under awaiting position, only because of its standard of English. In this phase editorial in Chief given...

29 June 2020 3,418 14 View

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

12 February 2020 9,022 3 View

Can Si acts as hole scavenger material for solar cell application?

If silicon is coated on hole conducting material, to stop the flow of holes in one of direction, can we use Si as hole scavenger material?

26 August 2019 185 2 View

Is anyone worked on machining of elastomer using abrasive water jet machining?

Is it possible to machine elastomer using abrasive water jet machining?

07 August 2019 5,635 7 View

What are the standard heattreatment temperature and conditions for inconel 718 alloy?

what are the standard heat-treatment temperature and conditions for Inconel 718 alloy? 1st homogenization (heat-treatment temperature and process ?) 2nd solution Heat treatment and aging? 3rd...

02 June 2019 4,278 4 View

Magnetic properties of HfO2

20 June 2017 1,834 2 View

How to deposit Graphene on HfO2 with good interface quality?

20 June 2017 1,468 2 View

How to etch HfO2 when it is deposited on graphene?

How to etch HfO2 when it is deposited on graphene?

26 April 2017 549 2 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

26 January 2021 4,071 3 View

I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

17 January 2021 5,516 3 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View