What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

How to characterize epitopes within a peptide sequence?

Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...

20 January 2021 2,201 1 View

How can I generate a population with a fixed correlation coefficient in R?

I want to generate an artificial population using two variables (say x & y) with a fixed correlation between x & y. Which package/command in R software can help in this?

18 January 2021 2,545 9 View

Please suggest ways to perform Discreet Element Modeling (DEM) on stabilized clayey soil; is it effective to simulate clay for DEM analysis?

I am looking for answer considering modelling in PFC

26 December 2020 5,509 1 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

What is the advantage of using AAV-DJ over native serotypes of AAV virus for tracing neurons?

I want to label forebrain neurons so as to trace specific (multiple) circuits. We have used AAV2/8/9 in our lab so far, and they have been pretty good. AAV-DJ seems to have more infectivity rates...

23 October 2020 9,838 2 View

What is the best feasible method to plot DISTANCE vs LST graph?

The above question is intended to be answered in reference to the reduction of the urban heat island by the river.

22 October 2020 7,732 4 View

How do different slots on patch helps in increasing the bandwidth of an antenna?

antenna designing

15 October 2020 1,788 4 View

What is the mathematical model of antipodal antenna design?

what is the mathematical expressions and equations used for the designing of antipodal structure of an antenna.

26 September 2020 7,948 3 View

Factor and Multivariate Analysis of Geochemical data (Generated from Geochemical Mapping using stream sediment samples)?

I want to know about any training module or step by step guide for doing factor analysis and multivariate analysis. This is required for analysing Geochemical data obtained from Geochemical...

24 July 2020 266 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View