How does nucleus size vary with concentration (in context of protein aggregation)? I understand that nucleus is formed faster at higher concentrations but how does number of molecules in the critical nucleus size vary with concentration?
According to classical nucleation theory, I have read that it increases with decrease in concentration. Can someone help me understand this?
Dear Nidhi,
In a classical nucleation dependent polymerization mechanism, the size of the nucleus does not change with protein concentration. In fact, the size of the nucleus is determined by obtaining various parameters (for example, t50 or lag phase) at different concentrations of monomer. You can see the paper and the references therein for more information:
https://www.ncbs.res.in/sitefiles/Sabaree_Udgaonkar_2014.pdf
I know however, that the mechanism of protein aggregation itself changes with increasing monomer concentration in some cases. But in those cases, what happens to nucleus size, I'm not sure.
Best,
Jogender
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