I have looked into a lot of papers but majority of them use either toxic chemicals (like electropolymerization of pyrrole and entrapment of GOD into the polypyrrole, crosslinking with glutaraldehyde) or long process (24h+ incubation before use).
What method is usually used in mass production of glucose sensors? It must be something like one or two steps drop coating. Or am I missing something here?
I have been trying to use various polymer matrices with carbon screen printed electrode but the repeatability of the measurements was very bad, the GOD seems to fly out of the working electrode during dipping of the electrode into buffered solution with glucose.
Dear Sir. Concerning your issue about method for immobilization of glucose oxidase and potassium ferricyanide on carbon screen printed electrode . A disposable amperometric biosensor for detecting blood glucose has been developed. The sensor is based on a screen-printed electrode prepared by electrochemical polymerization of pyrrole with glucose oxidase (GOD) and LiClO4 dopants. In citric acid buffer (pH5.0), GOD with negative charges is immobilized within electropositive polypyrrole matrices onto a carbon electrode surface. Afterward, the electron transfer mediator, potassium ferricyanide is immobilized by adsorption. Experimentally the compositions of pyrrole, LiClO4 and potassium ferricyanide are optimized. Amperometry is used for the determination of glucose concentration. Four microliters of glucose solution is needed for one test, and the response time of the sensor is 70s. The amperometric response of the enzyme electrode is linear in the range of 1-30 mM. I think the following below links may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pubmed/17282595
http://cdn.intechweb.org/pdfs/16428.pdf
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.465.4529&rep=rep1&type=pdf
Thanks
You want to immobilize GOx directly onto the SPE ? or first use the nano-materials as support matrix for GOx? in both cases you can use Chitosan as biding agent. First mix the GOx and nanomaterials solutions together and then inject the chitosan solution to it. Drop cast this dispersion solution onto the SPE which will make a stable film. However, there is a loss of conductivity due to chitosan but not significantly.
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