I would be starting a colony of susceptible and resistant strains and testing for pesticide resistance.
See attached our protocol for the codling moth articificial diet.
Frantisek
I reared a Tortricid (Lobesia botrana) with artificial diet, I can send you the ingredients and rearing cages I used for this moth if you want
Dear Riaz,
We keep a codling moth colony on an artificial diet since 2002 - for details, see:
Fuková I., Nguyen P., Marec F. (2005) Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes. Genome 48: 1083-1092. DOI: 10.1139/G05-063
I attach our protocol for rearing and also a protocol for preparation of the articial diet.
Hope it helps.
Good luck in your research!
Frantisek
See attached our protocol for the codling moth articificial diet.
Frantisek
You can find more information about rearing codling moth colony in this book: World crop pest: Tortricid Pests, 1st Edition, Print Book ISBN :9780444880000. Also you can buy some ready-mix diet from Southland Products: http://www.tecinfo.com/~southland/pricelist.html
It depends of the purpose. You have to start with collecting larvae of first generation and to hold them in condition of 18 h laight to select larvae which will develp without diapause. If you do not want changing the quality of the moths it will be better to use unripe apples.
For example, a strain resistant to diflubenzuron in codling moth should be maintained with sublethal doses of insecticide.
The Stonefly is a standard diet for bioassays. The artificial diet Stonefly Industries Ltd., Bryan, TX, USA.
There are two methods for rearing codling moth in the book Handbook of Insect Rearing Vol.II edited by Pritam Singh and R.F. Moore (published by Elsevier in 1985). They provide very detailed information on both the methods and diets to use. The FAO also has a handbook for rearing it for use in sterile insect programs (see attachment)
There are very easy resistance detection test developed by Charmillot - Detection de la resistance du carpocapse Cydia pomonella -Charmillot, P. J., D. Pasquier, Ch. Salamin, F. Briand, Ter-Hovannesyan, A. Azizan, H. Kutinkova, P. Peeva, N.Velcheva.
Revue suisse de viticulture arboriculture horticulture , Novembre-Decembre, 2007, vol 39, 6, 385-389 ( in French)
Our unit rears large numbers of codling moth on a regular basis. You may find information on our rearing procedures at:
http://www.ars.usda.gov/Services/docs.htm?docid=20109
Look down for the species list and click on "codling moth" for a pdf that detail the diet and methods used.
Because the larvae are cannibalistic, they are reared in small diet cups, only 2-3 per cup. There is a fair amount of labor involved in rearing this insect.
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