Fares Bellameche

4 Questions 7 Answers 0 Followers

Questions related from Fares Bellameche

Has anyone a protocol to perform Biparental mating using the plasmid pMF230 (E. coli ) ?

Dear researchers, I am trying to tag a Pseudomonas synxantha, I got the plasmid of gfp pMF230, but looking at the articles used the same plasmid, I didn't find much information how they perform...

30 July 2024 2,292 8 View

Is this Sybr stain for gel good enough ?

Hello, I usually use Invitrogen™ SYBR™ Safe Gel Stain for visualization of DNA in agarose gels (which works perfectly). But since I found this product : 1 ml ClearLine® EmeralDye Stain from...

17 May 2024 7,195 0 View

What is the easiest and cheapest way to get your strain tagged with gfp or mCherry ?

Dear researchers, Lately in our lab (Unimore, Italy) we wanted to tag a Pseudomonas strain (Biocontrol) with gfp or mCherry in order to use it for localization assay, please could you help us,...

01 March 2024 5,981 3 View

what is the best molecular approach for a bacterial strain-level detection?

Hello scientists, we isolated in our lab a nice promising biocontrol agent (Bacteria), now in field application, we need to re-isolated it and somehow make sur is the right one, we are thinking...

16 February 2024 8,982 5 View