What is the dynorphin level in human serum and plasma?

Bardia Varastehmoradi @Bardia-Varastehmoradi

28 February 2019 0 9K Report

I would like to measure the level of dynorphin in human and rat blood samples (mostly in serum blood samples).

How can I measure accurately Dynorphin in human blood samples? What are the different methods in animal experiment and clinical human study?

In advance, thanks for your suggestions.

Badges
Science topic

More Bardia Varastehmoradi's questions See All

Hi everyone, I am looking for insights into the main cost components of data collection?

Specifically, I am interested in understanding which parts of data collection are most costly. Is it the data collection frequency, the level of expertise required, the data collection methods,...

04 June 2024 5,377 5 View

How to solve the agglomeration problem with exosomes?

Hi everyone. I am having trouble with my exosomes since they have a high tendency to aggregate, as observed in DLS data and FESEM images (size ranges from 200 nm to 2 micrometer). I have tried...

03 March 2024 9,758 1 View

How do we precipitate a certain plasmid using Poly Ethylene Glycol (PEG)-MgCl2 Method (A problem in optimization) ?

Hello We have a problem in precipitating plasmid DNA (Exactly PIRES2-EGFP) with the PEG-MgCl2 method. The mentioned plasmid after culturing in E.Coli bacteria, harvested and extracted via using of...

11 March 2021 9,659 0 View

Do you kow any biomarker testing laboratory to detect biomarkers related to kappa opioid system?

Hello I am interested to study opioid biomarkers in human blood, especially in kappa opioid system. My main targets are dynorphin and oprk. since the amount of our samples are limited I would...

14 February 2021 3,864 0 View

Multi band on western blot for glucocorticoid receptor. Which band(s) should I select for quantitative study?

Hello I can see different bands for phosphorylated glucocorticoid receptor protein on the blot membrane. I get confused which band(s) should I select? Can I select the whole areas where the bands...

17 August 2020 6,975 8 View

Is it possible to have machine learning techniques in realtime systems?

Machine Learning (ML) is used to exploit some kind of predictability in the system, while in realtime systems you have to know the exact values and their isn't any place for probabilities. So, do...

07 March 2019 2,171 3 View

What is the best method to isolate amygdala from the fresh brain in rats?

I would like to isolate hipocampus and amygdala in rat. what is the best way that I can do it with the least tissue contamination?

25 February 2019 6,769 3 View

Is it possible to multiply more that 2 fuzzy numbers? If yes, what is the principal and also its Matlab function?

There are some methods for multiplication of two fuzzy numbers. But if we want to multiply more that two fuzzy numbers, how we should perform?

21 February 2019 8,034 6 View

Is there any rules or suggestions for defining the range of common areas (please check the attached photo) in the fuzzy diagram?

Evidently, the bigger the common area, the greater the uncertainty and vice versa.

10 January 2018 5,061 5 View

What are the most important criteria that sewer network decision-makers should consider during the management planning of these networks?

In addition to the Risk of failure, that consist of the probability and the damage consequences, such as repair cost.

01 June 2017 4,586 5 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View