There are huge references about ES but I couldn't find some good publication about embryonic stem cell derived mesenchymal stem cell (ES-MSC).
Also what is the differences between ES and ES-MSC in isolation and cell therapy?
To my knowledge, EC can develop into various types of cells, whyle EC_MSC can only develop into soft tissue associated cells.
W
Dear Dr. Wolfgang Doerr
I appreciate your taking the time to answer my question.
I know we can isolate ES from Inner Cell Mass (ICM) of blastocyst stage, but how can we obtain ES-MSC?
You can obtain adult pluripotent subpopulations within MSCs such as SSEA-3 or SSEA-4 positive cells by cell sorting. However, these cells called adult pluripotent subpopulaions not ES-MSCs.
Hello
I agree with Ali M. Fouad,
These links will help you
Article Human Embryonic Stem Cell-Derived Mesenchymal Stroma Cells (...
Article Mesenchymal stem cells: A new trend for cell therapy
Good Luck
Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...
04 August 2024 3,461 3 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View
Hi Dear All I would like to simulate a mode locked semiconductor laser. Does anyone know what is the best software to do it?
06 July 2024 1,648 4 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View
How can rural business building help to improve rural economy?
15 June 2024 3,462 3 View
For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...
04 June 2024 4,497 0 View
Hello all, I am doing some heavy simulations in Fluent using clusters. The models are not readable by laptop or desktop because they are very heavy. So, i have to take the contours in cluster...
25 May 2024 6,099 3 View
If we want to obtain Aerogel from Hydrogel for Oleogel development, does the syneresis of Hydrogel affect the future oil absorption process in Aerogel? Does the volume reduction due to syneresis...
17 May 2024 9,141 1 View
Hello every one. I want to simulate a drug through membrane and i need to gain structure and topology file of the drug molecule . i used to do this by using Prodrg under-web program but...
15 May 2024 5,439 0 View
Additionally, I welcome any general advice and suggestions you may have.
11 May 2024 5,419 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View