What is the difference between water-soluble and regular cholesterol?

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Kajanthan Piraisoody

Water-soluble cholesterol consists of both cholesterol and methyl-b-cyclodextrin. Since cholesterol is lipophilic, in physiological conditions, it is transported in the blood using Albumin. Methyl-b-cyclodextrin is a sugar derivative that is water-soluble and with a high affinity for cholesterol. Hence, this agent is highly suitable to solubilise cholesterol in water. In general, a very tiny fraction of the cholesterol in plasma is free due to its hydrophobicity.

Adil Maqbool

Hello Giora,

What i found that:

Water-soluble cholesterol vs regular cholesterol: Regular cholesterol is a waxy, fat-like substance that's found in all the cells in your body. It is hydrophobic and does not dissolve in water. On the other hand, water-soluble cholesterol is a form of cholesterol that has been modified to be soluble in water. This is typically achieved by attaching a hydrophilic group to the cholesterol molecule, which allows it to interact with water molecules and dissolve. This modification does not change the basic structure of the cholesterol molecule, and it can still be recognized and processed by the body in the same way as regular cholesterol.

Measurement of cholesterol in plasma: Cholesterol in plasma is typically measured using an enzymatic assay that involves the enzyme cholesterol oxidase. Even though cholesterol is not soluble in water, it can still be processed by enzymes in an aqueous environment. This is because enzymes like cholesterol oxidase are able to interact with the hydrophobic parts of the cholesterol molecule and catalyze a reaction that converts cholesterol into cholest-4-en-3-one and hydrogen peroxide. The hydrogen peroxide can then be detected using a colorimetric assay, which provides a measure of the amount of cholesterol in the plasma.