Olympus CKX41 inverted microscope (is it possible to replace the mechanical stage by a motorized one)?

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What is the difference between water-soluble and regular cholesterol?

Can anyone explain the difference between water-soluble cholesterol (sold by Sigma, C4951) and regular/natural/chemically pure cholesterol, which must be insoluble in water? How can we measure...

12 June 2023 391 2 View

What does "minimally cross-linked SU-8 photoresist" technically mean?

Hello everyone, In the SU-8 3000 specs it is claimed that "To remove minimally cross-linked SU-8 3000, or when using Omnicoat, heat the Remover PG bath to 50-80°C and immerse the substrates for...

01 June 2022 6,762 0 View

How to implement the autocollimation technique in the case of a pulsed femtosecond laser?

Hello everyone, Normally, I use the autocollimation technique in the case of a cw laser which implies the use of an additional objective lens (OL) right away the laser to wider the beam. However,...

08 May 2021 9,216 1 View

How to reduce transient time when generating nanosecond pulses with a Pockels cell?

I try to generate nanosecond pulses with a Pockels cell. The experiment scheme is: The laser beam, passing through the optical isolator, enters the Pockels cell, then through the Glan-Thompson...

24 March 2021 7,371 1 View

There is an opinion that the relativity principle plays a minor role in the GR, Is it so?

The principle of relativity is important for the special theory of relativity. In the general theory of relativity, it is read unimportant. The preference is given to the covariance of the...

26 March 2019 194 56 View

Where can I find work related to the theory of Nordström?

I'm working on a new version of GR. Help please find work related to the theory of Nordström. Particularly interesting are the results of Nordström's results of the gravitational theory. From...

20 April 2017 5,394 4 View

What technique do you prefer to make permanent modern pollen slides?

We treat specimens (anthers) with 10% KOH and then mount them in glycerol, but there are some articles where authors say that glycerol gradually destroys pollen grains. What do you think?

21 April 2015 7,001 18 View

Who can suggest a set of protease inhibitors to trace the intracellular degradation of recombinant protein in the transfected cells?

The goal is to identify if the recombinant protein is degraded by the proteasome or not. And if not, try to clarify which protease is involved.

21 February 2013 7,902 5 View

What has been done in the theory of gravity over the past 100 years? The answer is NOTHING.

The only confirmed theory of gravity is the general theory of relativity. It is confirmed by the results of observations. In particular, it has been confirmed that our Universe is described by...

01 January 1970 6,056 55 View

Another solution to the gravitational field equation

Alexander Zavesov proposed a new solution to the field equation, in my work New version of General Relativity and the Structure of "Black Holes" this is an exact imaginary solution s=A r^(sqrt(6)...

01 January 1970 3,779 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View