How to purify cyclic peptides?

Hello, We have synthesized the cyclic peptide (D-Glu(OtBu)-L-Lys(Alloc)-D-Glu(OtBu)-L-Ala)2. LC-MS shows a mixture of linear and cyclic peptides. There is only one peak by HPLC (reverse phase,...

21 July 2020 2,237 1 View

Can someone arrange me or indicate where I can find an IR solar panel data set?

I am in a master degree research and I'm in need of a data set for image processing of solar panels with defects (mainly hot spots), so I need to know if someone can help me and tell me where I...

06 January 2020 6,758 3 View

Does the concentration of the extract influence the GC/MS analysis?

how the concentration of the extract analyszed can influnece on the quality of the GC/MS analysis. Should I use low concentrations or high concentrations?

14 January 2018 8,209 5 View

When using RDA, why does my explanatory matrix explain a large amount of variation in my response matrix, but is not significant?

15 March 2017 5,983 3 View

COVID 19 :mRNA vaccines. and autoimmune phenomena/diseases.

BACKGROUND. I am not a molecular biology expert. I am a retired rheumatologist who dealt with autoantibodies and autoimmune diseases. Rheumatology has been caught up in COVID-19 business via...

01 January 1970 5,133 10 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to Remove different version of atom from pdb Crystal Structure for Docking Preparation?

I am new to Docking and MD, and currently try to catch on it. I try one of the webserver for docking, but unfortunately not working. it says " Your PDB contains multiple forms of the same residue...

08 February 2021 7,426 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View