need answers for the above question
Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...
13 June 2024 2,961 1 View
The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...
10 December 2023 5,680 2 View
I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...
18 February 2022 10,013 2 View
While generating a sequence identity matrix in bioedit software by pasting the sequences from notepad, often there is an overlapping of sequence names (given before the fasta sequence) and the...
10 January 2020 1,339 0 View
I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...
16 December 2019 7,151 5 View
I am going to do my research on Zero Shot Learning. Is it good area in recent trends...
03 December 2018 7,235 3 View
plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?
14 April 2018 6,544 2 View
If there is an accidental ethidium bromide spill on skin/hands during a moelcular work what is the immediate first aid measure to be taken using very simple chemicals available in the lab?
12 March 2018 443 3 View
Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...
09 March 2018 3,232 4 View
Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...
17 February 2018 667 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
