Different amplification strategies work better than others depending upon the templates, primers, and target sequence in amplification. Unless you know through empirical data you will not know if nested or semi nested primers give the better results. In nested PCR you use outside primers for first round(s) of amplification and inside primers for second round of amplification. In semi-nested PCR you use outside primers for first round(s) and one inside primer and the other previously used outside primer for the second round of amplification.
hello
In nested PCR you use outside primers for first round(s) of amplification and inside primers for second round of amplification. In semi-nested PCR you use outside primers for first round(s) and one inside primer and the other previously used outside primer for the second round of amplification.
https://www.researchgate.net/post/What_is_the_difference_between_nested_and_semi-nested_PCR_in_molecular_detection_of_parasites
hello
In nested PCR you use outside primers for first round(s) of amplification and inside primers for second round of amplification. In semi-nested PCR you use outside primers for first round(s) and one inside primer and the other previously used outside primer for the second round of amplification.
https://www.researchgate.net/post/What_is_the_difference_between_nested_and_semi-nested_PCR_in_molecular_detection_of_parasites
Hi
I think the different amplification strategies work better than others depending upon the templates .
regards
Different amplification strategies work better than others depending upon the templates, primers, and target sequence in amplification. Unless you know through empirical data you will not know if nested or semi nested primers give the better results.
Can you please elaborate how we can do seminested PCR. I mean what can be its profile order.
I need more clarification on semi-nested PCR. Does it mean 2 primers will be used in the second PCR?
Nested... yes, two different primers than in the first round.
Hemi-nested... no, one new primer NOT USED in the first round and one primer USED in the first round. I provided a visual to help clarify, hopefully.
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