21 April 2023 0 4K Report

research project on antibacterial activity of lavender essential oil , i have run into some issues with 96-well plate microdulition method for determining of minimum inhibitory concentration (MIC)

We use 160 ul of broth that contain 5%DMSO

20 ul of bacterial suspension

20 ul of essential oil , by two fold serial dilution

Oil initial volume 12.8 v/v

Reading result by micro plate reader at 600nm

Optional density Staphylococcus aureus is 1.223 - 1.102 -1.334 - 1.38- 1.625 - 1.639

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