research project on antibacterial activity of lavender essential oil , i have run into some issues with 96-well plate microdulition method for determining of minimum inhibitory concentration (MIC)
We use 160 ul of broth that contain 5%DMSO
20 ul of bacterial suspension
20 ul of essential oil , by two fold serial dilution
Oil initial volume 12.8 v/v
Reading result by micro plate reader at 600nm
Optional density Staphylococcus aureus is 1.223 - 1.102 -1.334 - 1.38- 1.625 - 1.639