I want to purify a protein of molecular weight less than 150 K.D using the gel filtration method, on buying the sephadex G100 there were 2 different types the medium & the super fine which one should I choose?
The best way is to check on GElifesciences (web site of the producer and distributor).
Choice of the material depends on what you want. The difference is simply the size of the dextran beads. "Superfine" material contains smaller beads. Thus, The diffusion distances in your column are shorter than in medium-sized particles. Consequently, the resolution of "superfine" is higher, separations between proteins is better for molecules with similar sizes. On the other hand, "superfine" columns need longer time. Normally, you will probably get along with "medium" material, which is also easier to run and less expensive.
Good luck!
Fully agree to Johannes! Fine answer! One additional note. Although the resolution of "superfine" is higher due to small size of dextran particles and faster establishing of equilibrium in concentrations of molecules diffusing into granules, with "superfine" column you will need to use slower elution rate to avoid deformation of dextran beads.
I would only add that the choice of bead diameter will depend on the your flow rate and this will depend on the size of your column. If you have a larger column you may want to use the larger bead to increase your flow to prevent diffusion from decreasing your resolution.
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