MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How to dissolve Pentachloronitrobenzene?

Pentachloronitrobenzene is used to prepare the "Nash & Snyder" media to isolate the Fusarium. But this is not dissolving in 75% ethanol and distilled water (Normal and hot both). So Please...

05 February 2021 687 1 View

How monolayer graphene can be grown on copper foil via LPCVD technique?

Please tell me about the rate (in SCCM unit) of the flow of gases and pressure inside the tube.

12 October 2020 8,315 3 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

Is there any mathematical model to study the electric fringe field for parallel plates having equal and opposite potential?

Some Mathematical expressions will be helpful.

26 April 2020 8,000 2 View

Which form of polyaniline (emeraldine base or emeraldine salt ) show antibacterial property and why?

The emeraldine form of polyaniline often refer to as emeraldine Base (EB) is neutral, if it is doped (protonated) with acid(1M HCl) it is called emeraldine salts (ES) is conducting, with imine...

24 February 2020 5,117 3 View

How do I resolve the following error I get during the execution of WRF gen_be for creating background error covariance file?

I am trying to generate the be.dat file using the gen_be facility of WRF. I have 12 hour and 24 hour outputs from WRF at 00 UTC and 12 UTC for a month. When I try to run gen_be_wrapper.ksh on my...

15 January 2020 244 2 View

Is there need to downscale CORDEX (RCM) data for our region? or Can we directly apply them after bias correcting them?

I have a query regarding data collected from CORDEX models and CMIP5 models. After reading about both, I have concluded that data from CMIP5 have to statistical downscale first then can apply for...

22 December 2019 2,655 5 View

Can a non-binary sensing matrix be implemented using a Digital Micromirror Device?

In compressive sensing of images Digital MicroMirror Device (DMD) is used to implement the sensing matrix. DMD works in two states, on and off. This can implement a binary sensing matrix. How is a...

05 November 2019 2,096 3 View

Embroid bodies formation from human iPSCs?

Do anyone know is it necessary to use low attachement plate instead can I use bacterological petri dish ?

10 October 2019 5,587 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View