Can anyone explain how to calculate BRI (Biomarker response index)?

Hello everyone, Can anyone tell me how to calculate biomarker response index (BRI)? Suppose I have three treatment sets and one control set of plants. I have considered many cytotoxicity,...

28 October 2020 9,304 3 View

Ideal sample size calculation for a descriptive study ?

I wish to estimate the expression of two types of markers by immunohistochemistry in the biopsy specimens of a particular type of cancer, and correlate their expressions with clinical parameters...

20 September 2020 9,662 6 View

What will be the impedance of a 75 micron diameter platinum-iridium wire?

I would like to know how does the impedance of a wire depend on its thickness. I want to make an implantable extracellular electrode for mouse brain, but I have to keep the wire thickness in mind...

11 February 2020 347 3 View

What are the reasons for action potential threshold to change between step current injections?

I am using HEKA in current clamp mode to record from mouse DRG neurons. I record action potential in DRG neurons by injecting 80 ms current pulses from 100 - 1000 pA in 50 pA steps (current step...

18 September 2018 3,044 3 View

I am not getting band for insert(3kb) upon digestion of plasmid(5.3kb) from colony PCR positive colonies. What might be the reason?

I am trying to clone a 3kb insert into a 5.3kb vector. The colony PCR was positive for around 12 colonies but upon digestion of the plasmid isolated from those colonies, no insert was observed....

15 December 2017 8,640 3 View

How can I make cross sections of honey bee brains?

16 May 2016 6,162 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View