What is the depth of layer 6 in mouse cortex?

More Abhrajyoti Chakrabarti's questions See All

Why is MAXQDA Pro 2024 is not analysing the documents uploaded despite of the codes being entered multiple times?

The screenshot is attached.

09 April 2024 8,756 0 View

BH curve from MH curve ?

I have the M-H curve (Magnetization vs field) for micron sized magnetic particles using which I prepare a suspension of a ferromagnetic fluid with a volume fraction of 40% in a non magnetic...

28 May 2022 2,638 2 View

Can anyone explain how to calculate BRI (Biomarker response index)?

Hello everyone, Can anyone tell me how to calculate biomarker response index (BRI)? Suppose I have three treatment sets and one control set of plants. I have considered many cytotoxicity,...

28 October 2020 9,503 3 View

Ideal sample size calculation for a descriptive study ?

I wish to estimate the expression of two types of markers by immunohistochemistry in the biopsy specimens of a particular type of cancer, and correlate their expressions with clinical parameters...

20 September 2020 9,937 6 View

How can we find the histogram plot for a 3D image?

I am working on 3D medical images I am unable to do histogram analysis of the image. Can anyone provide me a link on how to find the plot for a 3D image?

06 May 2020 8,761 0 View

What will be the impedance of a 75 micron diameter platinum-iridium wire?

I would like to know how does the impedance of a wire depend on its thickness. I want to make an implantable extracellular electrode for mouse brain, but I have to keep the wire thickness in mind...

11 February 2020 500 3 View

Computing marginal effects after -cmp- command in STATA?

(posted in statalist as well with no answer) I am trying to find marginal effects from a system of equations that I estimated using an user written command -cmp- (Roodman, 2009) in STATA. I have...

10 April 2019 6,721 2 View

What is the significance of applying two parameter Weibull distribution equations for evaluating the dielectric breakdown strength (DBS) of samples?

For calculation of DBS of glass-ceramic samples, a two parameter Weibull distribution is applied in 99% cases. Xi = ln(Ei) ; Yi = ln(-ln(1-i/n+1)) where Ei = specific breakdown field of each...

12 December 2018 9,841 0 View

What are the reasons for action potential threshold to change between step current injections?

I am using HEKA in current clamp mode to record from mouse DRG neurons. I record action potential in DRG neurons by injecting 80 ms current pulses from 100 - 1000 pA in 50 pA steps (current step...

18 September 2018 3,184 3 View

I am not getting band for insert(3kb) upon digestion of plasmid(5.3kb) from colony PCR positive colonies. What might be the reason?

I am trying to clone a 3kb insert into a 5.3kb vector. The colony PCR was positive for around 12 colonies but upon digestion of the plasmid isolated from those colonies, no insert was observed....

15 December 2017 8,793 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View