What is the decontamination protocol against Mycobacterium?

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Is there a direct connection between zero core reluctance and zero leakage flux?

I have seen that some lecturers claim the leakage flux of a transformer will automatically become zero in case it has an ideal iron core. I want to say NO! This is correct for a system with a...

03 May 2024 4,010 14 View

Can engineers help me answer the questionnaire?

I need help with one survey to reach 3000 people (response). I will be really thankful if anyone can reply and share this...

01 February 2024 1,437 7 View

Do you use the hardness of pomegranate peel to determine the harvest date?

The hardness of the peel of pomegranate fruits is affected by irrigation, the environment, and the number of fruits on the tree, so it is difficult to rely on the hardness of the peel in...

10 January 2024 3,609 1 View

How can I make a buffered nutrient solution which be susceptible in alkaline environment?

I want to make a nutrient solution. When I use it in alkaline soils, some of elements such as P, Fe, Zn, Mn, Cu will change to non soluble salts which are not available to plant. How can I make a...

26 October 2023 6,655 0 View

How the water increases the Concrete Slump?

Dear Sir, We all know that the water cement ration 0.45, which may vary due to the Mix-Design. But during the concrete due to changes of weather & materials SSD condition the slump changed...

12 October 2023 3,379 6 View

What are the parameters using for synthesis nanoparticles in liquid using laser ablation?

please i would like to collect all the parameters related to the synthesis of nanoparticles using laser ablation, in terms of laser parameters, liquid parameters, environment parameters and if any...

27 September 2023 5,713 7 View

What is the Effect of container diameter on nanofluid stability?

Long time a go, I came across a journal paper that describes the effect of vial or container diameter on the dispersion stability of nanofluid. It mentioned that, if three vials had the same...

25 August 2023 5,261 3 View

How to model CFRP?

Hello, I have a study of columns strengthened with 2, 4, and 6 layers of unidirectional CFRP. 1)- Does the number of layers refer to the number of plies, or should I divide each layer into 2...

28 July 2023 7,659 2 View

How can receive Li-Fi use smart phone camera?

In Li-Fi technology, information is sent by an LED and a receiver receives the information The question is, that its possible to receive information through a smartphone camera without an external...

09 July 2023 1,319 0 View

How to find the intensity of a surface plasmon mode?

Hi everyone. I wanted to know how I can numerically calculate the intensity of a surface plasmon mode in photonic crystal fiber.

05 July 2023 6,488 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What type of contaminant is it and how to decontaminate?

Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...

08 June 2024 6,797 5 View

I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

22 May 2024 1,334 0 View

How can I improve Gibson Assembly's efficiency?

In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...

14 May 2024 9,861 3 View

FAD containing Protein purification?

I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....

16 April 2024 6,912 1 View

Dear researchers, which is the difference between an integrative and a replicative plasmid?

I am performing a bacterial transformation on Mycobacterium abscessus spp. abscessus using pMSP12::mCherry. I need to know if this plasmid is integrative or replicative

01 April 2024 8,366 3 View

How to eliminate a recurring mold contamination in the CO2 incubators?

Dear researchers, We are struggling with neverending mold contamination in all of our CO2 incubators. In the past, we used to do H2O2 decontamination/24hr-UV mode every two months now we do it...

04 January 2024 3,717 4 View

Regarding resazurin assay?

I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...

01 January 2024 7,916 2 View

How do we know the probe targets of GenoType NTM-DR kits?

I would like to know the species-specific probe targets of GenoType NTM-DR for mycobacteria. I need this information to compare with the sequence-based...

29 November 2023 7,215 0 View

Which of the following gram-positive bacteria causes pharyngitis (sore throat)? A-Neisseria B.Streptococcus C. Staphylococcus D. Mycobacterium?

Bacteriology

24 October 2023 7,903 10 View