I need a good positive control i.e. extremely sensitive. I have tried a few -some considered sensitive like HCT116 and Hke3 but I wanted a more sensitive control. I check for apoptosis after 72h.
Thanks in advance
Hi Rita L Fior...
I think 5FU induced G1 arrest not apotosis.
I used HCT116 and HT-29 cells for 5FU teatment and I treated 40ug/ml for 48 h.
It strongly induced G1 arrest not apoptosis.
If you want to see cell death you may use more than 40ug/ml, I recommend HCT116 cell for your experiments.
If you want, let me know I can show you the morphology chages and cell cycle data for 5FU treated HCT116 and HT-29 cells.
THANKS !!
Now HCT116 is working much better!
thanks again
rita
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