I need to dissolve some short peptides in HFIP, but I donot know how to prepare HFIP solution.What is the medium, and in what conc. , from solid HFIP.
Hi,
HFIP is a liquid and can be obtained from chemical suppliers, such as Sigma Aldrich (product number 105228). It is highly polar and miscible with water so can be diluted to prepare a solution (a few percent of HFIP in water, for example) that will dissolve peptides.
Good luck!
Typically, neat HFIP is used. This provides the best solvation conditions for peptides. The HFIP then can be diluted with buffer, or dried off to yield a peptide film, which then can be solvated with buffers. It all depends on the peptide protein you're using.
Thanks David, I have to dissolve Fmoc Val-Ile-Ala , in HFIP, would it be possible?
What's on the end of Ala? Is it an amide or acid? Also, what are you going to do with this tripeptide?
It is Acid at the end of Ala, I intend to study it's aggregation propensity. I am interested in Amyloid beta aggregation.
Why have you left the FMOC on the N-terminus? This doesn't seem to me to be particularly "natural." If you want to study aggregation, why not simply use and Acetyl-VIA-NH2 peptide? Regardless, this peptide is going to be difficult to study due to its non-polarity. You might try neat DMSO. You then can dilute out the DMSO with a second solvent. If you're working in a biologically relevant system, e.g., in Tris, PBS, et al., you could dilute by a factor of 20, making the DMSO concentration 5%, and see if the peptide precipitates.
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