Does anyone know the composition of the buffy coat? I mean the percentage of B cell, T cells, and Neutrophil in the buffy coat? I have googled for a looooong time but cannot get the answer.
You can find the information in the guide to the preparation use and quality assurance of blood components from the EU Committe on Blood Transfusion 17th edition in chapter 4 Centrifugation of Blood Components. The cells are primarily separated in function of their respective size and density. For a typical whole Blood volume of 450 ml, 60 to 70 ml of anticoagulant and if no initial filtration of the whole blood occurs, the BC composition will vary with the centrifugation speed as follows:
At low speed BC will contain 60 to 70% of the total leucocytes contained in the whole blood, with HCT 50-70%, 10-15% of the red cells and 10-25% of the platelets.
At high speed of centrifugation Hct will be 40 to 60%, red cells 10 to 15%, Platelets 80 to 90 % and leucocytes 50 to 70%.