What is the composition of M9 medium to isolate chitinolytic bacteria?

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Loc B Trinh

This is according to the "Labrat.com" which I had used.

Make M9 salts

To make M9 Salts aliquot 800ml H2O and add

64g Na2HPO4-7H2O

15g KH2PO4

2.5g NaCl

5.0g NH4Cl

Stir until dissolved

Adjust to 1000ml with distilled H2O

Sterilize by autoclaving

Measure ~700ml of distilled H2O (sterile)

Add 200ml of M9 salts

Add 2ml of 1M MgSO4 (sterile)

Add 20 ml of 20% glucose (or other carbon source)

Add 100ul of 1M CaCl2 (sterile)

Sathish Kumar

Mr Trinh... Thanks for your kind response..! Pleasure!