I need to isolate chitinolytic rhizobacteria, so I wish to know the exact composition of the medium used to isolate it.
This is according to the "Labrat.com" which I had used.
Make M9 salts
To make M9 Salts aliquot 800ml H2O and add
64g Na2HPO4-7H2O
15g KH2PO4
2.5g NaCl
5.0g NH4Cl
Stir until dissolved
Adjust to 1000ml with distilled H2O
Sterilize by autoclaving
Measure ~700ml of distilled H2O (sterile)
Add 200ml of M9 salts
Add 2ml of 1M MgSO4 (sterile)
Add 20 ml of 20% glucose (or other carbon source)
Add 100ul of 1M CaCl2 (sterile)
Mr Trinh... Thanks for your kind response..! Pleasure!
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