Sathish Kumar

4 Questions 13 Answers 0 Followers

Questions related from Sathish Kumar

Can anyone suggest the correct protocol to remove the bound DNA from the 6x His Tag protein as well the same protein without tag?

Whenever I purify the His tag protein plasmid DNA is bound with it & troubles in assays, though purification with His trap & anion exchange column was done, yet DNA is bound to protein....

12 December 2014 4,303 6 View

Where can I get Mycobacteriophage D29 DNA for research work?

I need to analyse the nuclease activity of mycobacteriophage D29, DNA template is required for the same approach.

11 November 2014 2,761 4 View

What is the composition of M9 medium to isolate chitinolytic bacteria?

I need to isolate chitinolytic rhizobacteria, so I wish to know the exact composition of the medium used to isolate it.

09 September 2012 6,482 2 View

Is there any cost effective alternative medium available to replace MS medium in Plant Tissue Culture?

Since in our lab we are mostly dependent on chemicals which are high priced, we wish to try an alternative for those chemicals. In need of suggestion to find an alternative to MS medium in Plant...

07 July 2012 8,371 27 View