The supreme property of chemical matter is its potency to have given rise to the emergence of life. Yet life’s origin continues to be one of the big unanswered questions of natural science. How prebiotic metabolic systems could have functioned in the absence of enzymatic networks is a critically important unanswered question about the origins of life. Such prebiotic cycles are considered as necessary platforms for the development of more advanced self-replicating biotic systems. The prebiotic reductive tricarboxylic acid (rTCA) cycle (also called the reverse Krebs cycle or the reductive citric acid cycle) has received much attention because it provides a core mechanism for the synthesis of useful biomolecules from CO2. Are there any reports or experiments conducted to test the hypothesis and complete the steps steps in a non-enzymatic manner?