Can lactase enzyme be used after breast feeding to baby or mixed with milk for others with lactase defficiency?

Lactase deficiency is not rare condition and baby with congenital lactase deficiency offers great problem to the parents. In the same way adult cases can't drink milk. If lactase preparation can...

03 April 2018 8,338 1 View

What is physiological basis of immunosupression during late pregnancy?

Immune-suppression is common during pregnancy especially during last trimester and there is every possibility of getting infection during this period. There is definite mechanism of this...

07 August 2016 3,608 1 View

Why different objects having same color show that color?

Different objects like a red peeper,red cloth, red blood showing same color with different chemical composition absorbing all color except red rays. But what is basic cause behind this property of...

08 September 2015 2,584 0 View

Does HbA1c always reflect past status of glucose level in diabetes mellitus?

Measurement of HbA1c is a good tool to monitor past (12 weeks) status of blood glucose level but it may sometimes misguide the physician. Recently a patient incidentally diagnosed as diabetes...

11 December 2013 438 12 View

What is the cause of halitosis (bad breathing) in diabetic patients?

Common cause of halitosis ( bad breathing) are oral or dental, respiratory or GIT problem or chronic sinusitis. Without any apparent infection, I found halitosis in many persons. Ultimately these...

09 October 2013 8,540 4 View

Does constant use of negative (concave) lens aggravate myopia?

Myopia is common among the students or persons particularly females who have a height less than 5 feet. Mostly its incidence is directly proportional with the shortness of height. We observed that...

08 September 2013 8,344 10 View

Would it be better to administer vaccines(e.g, MMR) through a natural (inhalation) route?

For a long time MMR or pertussis vaccines have been claimed to be associated with autism in children. When MMR or the pertussis vaccine are given intramuscularly, it bypasses a natural barrier...

31 December 2012 1,204 6 View

Why are allergies more common in developed countries than developing countries?

It is now established that allergy is more common in developed countries than developing countries and similarly it is more in urban than rural area.

10 November 2012 7,619 97 View

Can we use goat blood instead of sheep blood for preparation of blood agar media?

Usually sheep blood and sometimes horse or rabbit blood is preferred for the preparation blood agar media. But in text books of microbiology goat blood is not advised for this purpose. In our area...

10 November 2012 2,415 15 View

A microscopic picture of a stool

The patient was suffering from chronic diarrohea. I could not identify the arrow marked organism, it might be related to the disease. I found this organism several times but in less than 1% cases....

08 September 2012 3,819 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View