What is the chemical composition for preparing preparing polyvinyl - lactophenol?

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Only one clear RNA band on gel?

The RNA quantity in nanodrop is about 1 microgm. 260/280 ratio is also around 2, still I am not getting clear bands on gel. What can be the reason?

30 December 2020 2,010 2 View

How can we ensure that bias is removed in Qualitative Research?

I want to know the various methods and how efficient it is from your experience to bring in objectivity in qualitative research

30 June 2020 1,092 0 View

Tree ring analysis of tropical species?

Hello RG Users! Could you please suggest the appropriate technique for the tree ring analysis of the Tropical hardwood species especially Shorea robusta. Thanking you.

07 May 2019 9,033 4 View

Nepal is known as a land of community forestry. Could anyone supports on measuring green economy in case of community forestry?

Green economy interms of community based forest management!

18 September 2018 3,259 12 View

Can anyone suggest a good or the best algorithm for object identification in video and image?

I need to implement in computer vision.

06 September 2018 7,134 6 View

How we could come up with dendrochronological analysis of shorea robusta, we want to calculate the age and growth trend?

Hello Dendrochronologist! We are working in the tropical forest management issues, in which we are stuck in finding the relationship between age and growth of Shorea robusta species. We would...

30 July 2018 512 9 View

Can I get some help with RP-HPLC analysis of plant stanol ester ? Thank you.

Sample preparation Analytical column

24 July 2017 354 3 View

Has anyone applied geometric morphometrics for analyzing morphometric and meristic characters of fresh water fishes ?

I am new in this concept. So kindly provide some concept papers, analytical papers etc.

28 August 2016 8,525 3 View

Any advice on my problem in transforming Agrobacterium GV3101?

I am transforming Agrobacterium GV3010 competent cells with the plasmid after Gateway cloning(after LR reaction) by electroporation but after transformation I am getting bacterial lawn in next 12...

29 November 2015 9,698 37 View

Have anybody used the zooImage software for image analysis of zooplanktons? Is it reliable in using it for image analysis?

Recently, I downloaded ZooImage software for image analysis of freshwater zooplanktons. I want to know if anybody has used this software and whether this zooImage software is reliable ?

06 December 2014 6,573 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

Hi dear researchers, do you suggest the best and shortest path for chemical synthesis and green synthesis of magnetite(Fe3O4)?

Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...

21 July 2024 2,476 2 View