Only one clear RNA band on gel?

The RNA quantity in nanodrop is about 1 microgm. 260/280 ratio is also around 2, still I am not getting clear bands on gel. What can be the reason?

30 December 2020 1,731 2 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Cloning clusters in streptomyces?

Is someone working with cloning big size biosynthetic gene cluster (>50kbp).Quite difficult to fish the whole cluster??? Any tricks so far?

23 July 2018 5,993 16 View

What are the possible ways for utilizing municipal waste in agriculture?

other than composting.

26 June 2018 6,682 18 View

What is the minimum sample size required to include four dummies in multiple regression?

We have collected population growth data of a carnivore from five provinces and would like build a multiple linear regression model (OLS) with two explanatory variables. We have 7 observations...

07 January 2018 4,118 5 View

Can planted forests provide multiple ecosystem services? and to what extent?

13 November 2017 5,708 15 View

How to compute multiple excited state dipolemoments in one TD job?

Hi, I am working on a molecule of 34 atoms trying to compute excited state dipole moment using density=all. I know I can choose a state of interest by using root keywork (root=3 for third...

04 May 2017 7,577 3 View

Which is better semiconductor ptype or ntype, in what way?

16 December 2015 8,440 3 View

Have anybody used the zooImage software for image analysis of zooplanktons? Is it reliable in using it for image analysis?

Recently, I downloaded ZooImage software for image analysis of freshwater zooplanktons. I want to know if anybody has used this software and whether this zooImage software is reliable ?

06 December 2014 6,551 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Is aligning mitochondrial and nuclear sequences inconsequential?

Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...

19 January 2021 5,895 2 View

How can I make unsupervised clustering of trees species using algorithms like HAC, Kmeans... in R?

I am working on the development of multi-species volume allometric equation from diameter (DBH). I would like to form groups of species with the largest possible volume-diameter correlations by...

19 January 2021 5,752 5 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

What is the classification of tree species by texture analysis technique on drone images?

Hi My goal is to distinguish forest tree species (in dense forests) with drone images. I did not get acceptable results using spectral features. Because the pictures were taken in the fall and the...

07 January 2021 7,494 3 View