Will programmable logic controllers (PLCs) be replaced by IOT devices as the latter become more reliable?

Will programmable logic controllers (PLCs) be replaced by IOT devices as the latter become more reliable?

29 December 2020 255 6 View

Tcl script in VMD?

Using a VMD script written by me (MOisoall.tcl) I try to visualize the behavior of HOMO in the course of an inversion of ammonia (Fig. 1), but the VMD script does not work correctly (Fig. 2)....

11 October 2020 2,310 2 View

Dear Ji Eun, could you contact me urgently about your Primavelans n. sp. please?

Did you finish the paper and publish? Hope you and your family are well! Best regards, Hans

07 April 2020 1,490 4 View

Which is the Risk of Long QT and Sudden Cardiac Death by Drugs Used to treat COVID-19?

Numerous drugs are used these days without in-depth evaluation of efficacy and safety for the treatment of life-threatening complications of COVID-19. Among these, an important number are known...

26 March 2020 816 17 View

Which pharmacological modulation of immune response to SARS COV-2 could reduce the incidence of acute respiratory distress syndrome?

Differences in immune responses to emerging corona viruses and the difficulties of developping vaccines contribute to a surge in ARDS in vulnerable populations, leading to acute shortages and...

16 March 2020 3,520 26 View

Does anyone have a review article or reference describing the acid stability of SPE C-18 cartridges?

I am trying to find sources which describe the instability of C-18 columns and SPE cartridges. I know there are a few like X-bridge BEH that are more stable down to pH2. In our hands a regular...

03 March 2020 7,704 2 View

What are the statistics of use for the automatically triggered, obligatory subjunctive in Romance, especially Spanish?

In the Romance languages, many syntactic contexts, especially in subordinate clauses, require a subjunctive mood on the verb, not leaving any romm for mood choice. In other contexts, however,...

16 February 2020 3,747 2 View

No title

19 October 2019 4,850 3 View

No title

09 October 2019 1,823 4 View

Which fundamental questions about physical reality are still open?

The origin of gravitation, the origin of electric charge and the fundamental structure of physical reality are resolved, but these facts are not yet added to common knowledge. Also the structure...

09 October 2019 2,254 20 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

.In my results the slope of curves got from Microplate reader is seems less (less than 1) and negetive some times. What is the reason for that?

In my research I'm enumeraring the florescence intensity (FI) Vs E-coli concentration in river water. I measure FI by Tecan I control 200 pro and E-coli by Membrane filtration.

03 January 2021 2,991 3 View

Is it better to normalize Nile red absorbance to Bradford assay or to cell number?

I stained the fat droplet inside the cell using Nile red and read the absorbance by a microplate reader. Which better to do, normalize the Nile red absorbance to Bradford assay or to cell...

12 December 2020 8,036 2 View

Assay with absorbance/fluorescence readout?

I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...

11 November 2020 7,645 3 View

Assessing CoP by means of 3D motion capture system?

Hello, I would like to measure the postural control/weight shift during step initiation, especially by mean of CoP. For equipment, I am considering using a 3D motion capture system rather than a...

08 October 2020 5,876 3 View

Should absorbance be between 0 and 1 for ELISA?

Hello, I am running a sandwich-type ELISA for which the readout is absorbance at 450nm. The ELISA measures protein binding which I am screening for compounds to inhibit. The 570nm...

05 October 2020 9,813 6 View

Can I use a single gain adjustment when comparing multiple plates for fluorescence reading?

We are using the resazurin dye assay, specifically its fluorescence assay, to measure the growth of our bacteria using a BMG Labtehc Fluostar microplate reader. We have so many samples that it...

05 October 2020 6,714 2 View