Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

How has the mysteries (surrrounding the initial death rates) of corona virus shed light on other possibilities of the K-T boundary extinctions?

Could there have been some subtle underlying, seemingly unidentifiable cause of death that combined with the closely related catastrophes (eg asteroid impact hypothesis and/or disruption in animal...

01 August 2020 3,798 5 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Could one suggest a common formula for Penicillium Chrysogenum spore counting by spectroscopy? and also its nm?

I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.

05 January 2021 4,308 3 View

Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

CFU treatment log reduction without control?

Hello all, I am trying to create a graph on excel to express -log10 reduction of treatment groups and CFU spores using the equation -log10(final concentration/initial). However, one of my groups...

28 November 2020 6,074 3 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

How to isolate Clostridium from mixed culture?

My Clostridium tyrobutyricum seems not producing spores. I incubated the Clostridium tyrobutyricum in 10 ml of RCM at 28 degrees Celcius for 5 days anaerobically, then heat the whole tube in 75...

25 October 2020 3,386 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View