What is the cheapest method of isolation of mycorrhizal spores from the soil?

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In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

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Eduardo Furrazola Gómez

The cheapest method is to isolate spores from the soil by the wet sieving and decanting method of Gerdemann and Nicolson (1963), I believe. You only need sieves, water, Petri dishes and a microscope. Of course, if you have a centrifuge hanging glasses to centrifuge the soil that is in the sieve with a concentrated solution of sucrose and obtain the cleanest spores, it is ideal

Michael Richardson

It depends on what you mean by 'mycorrhizal spores' - ecto - and endomycorrihizal fungi are completely diffferent things. Eduardo's suggestion sounds pretty good for the endos.

Okon Okon

Thank you very much for your contributions..