How can i calculate sample size for a study?

sample size calculation

03 April 2018 6,698 0 View

How calculate sample size for genetic studies?

sample size calculator

02 March 2018 9,418 0 View

How you calculate additive model using SPSS?

if u have three genotypes GG GA AA How u calculate GG VS. GA VS. AA

11 December 2017 2,127 0 View

Haplotype frequency calculation

05 June 2017 5,276 12 View

Quality score of study

How calculate quality score of the case control study?

05 June 2017 184 0 View

In meta-analysis when I stratified my data according to ethnicity, which model i used fixed or random model?

In meta-analysis when I stratified my data according to ethnicity, which model i used fixed or random model?

05 June 2017 8,817 0 View

How can I use haploview to calculate haplotyptes and the link of this software if possible?

How can I use haploview to calculate haplotyptes and the link of this software if possible?

05 June 2017 1,453 1 View

Bonforoni correction

What is bonforoni correction of p value and how can i calculate?

05 June 2017 4,494 0 View

What is the difference between genomic DNA, Nuclear and mitochondrial DNA?

Is genomic DNA the same as nuclear DNA IS genomic DNA (nuclear + mitochondrial? the protocol for isolation of mitochondrial DNA the same as genomic DNA

04 May 2017 4,271 1 View

In genetic studies, what is the difference between alleles and haplotypes? and how can you calculate haplotype with example ?

how can you calculate haplotype with example ? difference between alleles and haplotypes

10 November 2015 2,481 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View