In many papers, PBS (pH = 7.4) is used to bind carbon dots and aptamers. Why?
Is it related to pH at which aptamer binds well?
Dear Hyeonjeong Jang sorry to see that your interesting technical question has not yet received am expert answer from RG members. In such reactions, phosphate buffer (PBS, = phosphate buffer saline) is added to maintain a physiological pH value of around 7.4 which allows for effective aptamer binding. In this context, please have a look at the following useful references:
Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis
Article Carbon nanoparticles with oligonucleotide probes for a label...
and
Multi-carbon dots and aptamer based signal amplification ratiometric fluorescence probe for protein tyrosine kinase 7 detection
Article Multi-carbon dots and aptamer based signal amplification rat...
I hope this helps. Good luck with your research and best wishes, Frank Edelmann
You may find following papers useful.
Article Visible light-induced charge injection and migration in self...
Article Creation of Linear Carbon Dot Array with Improved Optical Pr...
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