I am in a dilemma if I would agree on using a 6months old zebrafish for a 30day sub-chronic toxicity exposure.
I am usually aiming for a 3-5month old zebrafish and my acute toxicity experiment was done on 4months old.
I am unsure if the 6months old would be significantly different already or they might already be too old?
Hoping for your insights.
Thank you!