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Questions related from Carl Angelo Medriano
Although the loading mass for NGS would be the same, I am just thinking if it matters if my DNA concentrations after purification varies a lot (e.g. 20 vs 200 ng/uL)?
22 January 2017 1,886 1 View
It is my first time handling plasma samples. For a standard, we need to use a human plasma that have been lyophilized. What is the proper dissolution for it and what is the solvent? How much of...
27 October 2014 4,681 1 View
We received a plasmid delivered by mail and performed transformation experiment following protocol. the plasmid is used is topo with 3.9kbp. we used AK plates for blue/white screening due to the...
06 September 2014 4,883 6 View
I was preparing a phosphate buffer and tried to add glucose. This was to prepare a buffer with an energy source for a bacterial study. After autoclaving, there was a distinct change in color...
31 July 2014 2,160 15 View
We are planning to resuspend a fungal culture with phosphate buffer and add an energy source to sustain life while removing other compounds in a medium that might interfere with the possible...
22 July 2014 4,503 6 View