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Questions related from Carl Angelo Medriano
I am in a dilemma if I would agree on using a 6months old zebrafish for a 30day sub-chronic toxicity exposure. I am usually aiming for a 3-5month old zebrafish and my acute toxicity experiment...
04 August 2021 2,737 0 View
In our aerobic wastewater treatment tank, there is growth of pink worms as shown in the picture. Any idea what those might be? Would it affect our reactor? How to remove and avoid this...
21 March 2017 5,437 3 View
Although the loading mass for NGS would be the same, I am just thinking if it matters if my DNA concentrations after purification varies a lot (e.g. 20 vs 200 ng/uL)?
22 January 2017 1,903 1 View
I am working on detecting different compounds present in my sample. I ran in using LC-MS (Agilent QtoF). I am only used to use Metlin as my database for searching compounds using my m/z. But now I...
08 January 2015 4,675 2 View
It is my first time handling plasma samples. For a standard, we need to use a human plasma that have been lyophilized. What is the proper dissolution for it and what is the solvent? How much of...
27 October 2014 4,688 1 View
We received a plasmid delivered by mail and performed transformation experiment following protocol. the plasmid is used is topo with 3.9kbp. we used AK plates for blue/white screening due to the...
06 September 2014 4,889 6 View
I was preparing a phosphate buffer and tried to add glucose. This was to prepare a buffer with an energy source for a bacterial study. After autoclaving, there was a distinct change in color...
31 July 2014 2,164 15 View
We are planning to resuspend a fungal culture with phosphate buffer and add an energy source to sustain life while removing other compounds in a medium that might interfere with the possible...
22 July 2014 4,508 6 View
I read that typically, TBE is used for DNA while TAE is for RNA electrophoresis but I saw some literatures using TBE in RNA electrophoresis.
25 June 2014 3,166 10 View
